Total Genomics Solution · Genome Assembly Services

Chromosome-Level Assemblies for Crop Science & National Research

From plantation crops and staple cereals to medicinal plants — we deliver platinum-standard, haplotype-resolved reference genomes purpose-built for breeding programmes, germplasm conservation, and government mandated biodiversity initiatives across Asia-Pacific.

>98%
BUSCO Completeness across delivered assemblies
Chr-scale
Hi-C scaffolded chromosome-level output
IF 41.4
Highest impact factor journal publication
Haploid+
Haplotype-resolved for polyploid genomes
Trusted byPublic Universities & Research InstitutesNational Agricultural AgenciesPlantation & Seed CompaniesPharmaceutical & Nutraceutical ProducersInternational Breeding Consortia

The Strategic Case

Why Your Programme Needs a
Chromosome-Level Reference Genome

From Markers to Mechanisms

Fragmented draft assemblies restrict your genomic selection models to low-density marker panels, obscure structural variants driving economically important traits, and prevent accurate pan-genome construction across accessions.

A haplotype-resolved, chromosome-scale reference unlocks the full spectrum of modern crop genomics — from GWAS with physical-position resolution to map-based cloning of disease-resistance loci, and regulatory compliance for biosafety dossiers submitted to national authorities.

For government research mandates — biodiversity inventories, indigenous crop preservation, GI product authentication — a platinum-standard assembly is both a scientific deliverable and an institutional asset with long-term policy value.

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High Repeat Content & Polyploidy
Oil palm, wheat, sugarcane, and banana genomes contain 60–85% repetitive elements and complex ploidy. Short-read assemblies collapse paralogous regions; our long-read + Hi-C pipeline resolves them to individual haplotypes.
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Breeding Programme Integration
Marker-assisted and genomic selection pipelines require dense, positionally accurate SNP maps. Chromosome-scale assemblies anchor markers to physical positions, eliminating interval uncertainty that limits selection accuracy.
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Regulatory & Publication Standards
High-impact journals (Nature Genetics, Nature Plants, Plant Cell) and NCBI/EBI submission standards require N50 ≥ chromosome scale and BUSCO ≥ 95%. Our assemblies are delivered publication-ready.
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Germplasm & Biodiversity Documentation
National collections and CBD/Nagoya-compliant access-and-benefit-sharing frameworks increasingly require sequence-level characterisation. Our assemblies provide the authoritative molecular record for deposited accessions.

Our Technology Pipeline

Four-Stage Platinum Assembly Workflow

An integrated, end-to-end pipeline combining the highest-fidelity sequencing platforms with expert bioinformatics — from DNA extraction advisory through to annotated, publication-ready genome packages.

01
Long-Read Foundation
HiFi & Ultra-Long Sequencing
PacBio HiFi reads (≥99.9% accuracy, 15–25 kb average) and/or Oxford Nanopore ultra-long reads (N50 >100 kb) provide the contig backbone. Repetitive elements, transposons, and segmental duplications are resolved at base-level accuracy.
PacBio RevioONT PromethIONHiFiasm
02
Chromosome Scaffolding
Phase Genomics Hi-C
Proximity ligation captures native 3D chromatin interactions across the entire genome. Contigs are ordered, oriented, and anchored to chromosomal pseudomolecules with >99% scaffold accuracy — the definitive standard for chromosome-level assembly.
Proximo™ PlatformHi-C 2.03D-DNA
03
Accuracy & QC
Polishing & Validation
Illumina short-read polishing eliminates residual indels and SNP errors. Comprehensive QC includes BUSCO v5 (Embryophyta/Poales lineage), QUAST contig statistics, k-mer completeness (Merqury), and repeat landscape profiling via RepeatMasker.
BUSCO v5Merqury QVRepeatMasker
04
Functional Annotation
Gene Prediction & Analysis
MAKER/BRAKER2 evidence-based gene prediction integrating RNA-seq expression data. Functional annotation via InterProScan, GO enrichment, and KEGG pathway mapping. Comparative synteny analysis against related reference species to validate assembly integrity.
BRAKER2InterProScanOrthoFinder

Proven Crop Expertise

Reference Assemblies Across Asia-Pacific's Priority Crops

Our team carries validated experience across the full spectrum of Asian strategic crops — from plantation commodities to subsistence cereals and high-value medicinal species.

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Oil Palm
Elaeis guineensis / E. oleifera

Haplotype-resolved chromosome-level assemblies successfully delivered for this heterozygous, repeat-rich (84% TE content) diploid genome — a flagship demonstration of our Hi-C scaffolding capability for plantation crop species.

Haplotype-resolvedBUSCO ≥98%Disease resistance
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Rice & Wild Relatives
Oryza sativa / O. rufipogon

SMRT-sequenced assemblies for cultivated and wild Oryza species, enabling population-level pan-genome initiatives and introgression mapping for stress tolerance QTL. Published in Communications Biology.

Pan-genome readyWild accessionsIRRI-compatible
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Cotton
Gossypium hirsutum / G. barbadense

Landmark allotetraploid assemblies (Nature Genetics, IF 41.4) resolving the A and D subgenomes of tetraploid cotton — a technically demanding polyploid assembly demonstrating sub-genome phasing capability.

AllotetraploidSubgenome phasedNature Genetics
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Medicinal & Specialty Herbs
Panax notoginseng & related spp.

High-value medicinal plant assemblies supporting biosynthetic pathway elucidation, GI product authentication, and intellectual property protection for herbal commodity exporters and pharmaceutical R&D.

Pathway miningGI authenticationMolecular Plant
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Cereals & Grasses
Barley, Sorghum, Sugarcane, Bamboo

Experienced in C4/C3 grass assemblies including polyploid sorghum and complex sugarcane genomes. Low-pass WGS population genotyping pipelines available as a cost-efficient complement to reference assembly projects.

Polyploid capableGBS integrationASEAN staples
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Horticultural & Orphan Crops
Durian, Mangosteen, Pandan & others

We specialise in first-of-species reference assemblies for ASEAN horticultural and orphan crops where no prior reference exists — providing the molecular foundation for breeding programme initiation and export market value-adding.

De novo assemblyNo prior referenceExport commodities

Project Deliverables

What Your Institution Receives

Publication-Ready. Submission-Ready. Programme-Ready.

Every genome assembly project is delivered as a comprehensive data package designed to satisfy journal peer review, NCBI/EBI database submission requirements, and internal breeding programme integration — without further processing.

Government institutions and research agencies receive a complete technical report structured to support project reporting, grant deliverables, and institutional data governance requirements.

Data Governance NoteAll raw sequencing data, assemblies, and annotation files are delivered exclusively to the commissioning institution. No data is retained, shared, or used for any secondary purpose without explicit written consent.
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Chromosome-Scale Assembly (FASTA)
Pseudomolecule-level scaffolds anchored to chromosomes. N50 reported at chromosome scale. Unanchored contigs provided separately.
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Full BUSCO & QC Report
BUSCO v5 completeness (single-copy + duplicated), QUAST contig statistics, Merqury k-mer QV score, and repeat landscape summary.
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Gene Annotation (GFF3 + CDS)
Structural and functional annotation files in standard GFF3 format. Protein FASTA, CDS FASTA, and GO/KEGG functional assignment tables included.
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Repeat & TE Annotation
RepeatMasker-based transposable element classification and soft-masked genome FASTA. Essential for downstream GWAS, eQTL, and epigenomics analyses.
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Hi-C Contact Map
Genome-wide proximity ligation contact matrix (.hic format) enabling 3D genome topology analysis and independent scaffolding QC visualisation.
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Technical Project Report
Comprehensive written report covering methods, QC metrics, assembly statistics, and bioinformatics workflow — formatted to support grant reporting and institutional documentation.
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Synteny & Comparative Analysis
Whole-genome alignment and synteny plots against related reference species for assembly integrity validation and evolutionary context. Provided as publication-quality figures.
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NCBI/EBI Submission Support
File formatting, metadata curation, and submission-ready packages for GenBank (NCBI) or ENA (EMBL-EBI) deposition — critical for journal acceptance at high-impact venues.
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Scientific Track Record

Published in the World's
Highest-Impact Journals

Nature Genetics · 2019
Impact Factor 41.4
Allotetraploid Cotton Genome Assembly

Landmark comparative genomics study of Gossypium barbadense and G. hirsutum resolving the A and D subgenomes of allotetraploid cotton — one of the most economically significant polyploid assemblies published to date.

↳ TGS bioinformaticians: Co-first & contributing authors
Molecular Plant · 2017
Impact Factor 16.7
Panax notoginseng Genome

First reference-quality assembly for this high-value medicinal herb, elucidating the ginsenoside biosynthesis pathway and providing the molecular foundation for quality authentication and breeding of this protected traditional medicine species.

↳ TGS bioinformaticians: Contributing authors
Communications Biology · 2020
Impact Factor 6.3
Wild Rice Genome: Oryza rufipogon

SMRT-sequenced de novo assembly of the primary wild progenitor of Asian cultivated rice, revealing the genomic architecture of key adaptation loci and providing a critical resource for rice improvement and germplasm utilisation programmes.

↳ TGS bioinformaticians: Contributing authors
Phase Genomics
Strategic Technology Partner
Exclusive Asia-Pacific Partnership

The Gold Standard in Hi-C —
Now Available in Asia-Pacific

Phase Genomics is the global leader in proximity ligation Hi-C technology and the inventor of the Proximo™ genome scaffolding platform — used in more chromosome-scale assembly publications than any competing technology.

Through our strategic partnership, Total Genomics Solution delivers Phase Genomics Hi-C capability directly to crop research institutions and government agencies across Southeast and East Asia — without the logistics, lead-time, or cost overhead of offshore sample shipment.

Direct access to Proximo™ Hi-C scaffolding — the same platform used by USDA, CGIAR, and leading crop genome initiatives globally
Local project management, ASEAN sample handling logistics, and customs clearance support for cross-border DNA shipments
Bilingual (English/Chinese) technical reporting available for China-based institutional collaborators

Start Your Project

Ready to Establish the Reference Genome
for Your Crop?

Whether you are initiating a national genome programme, supporting a breeding institute's molecular platform, or building the reference resource for a published genomics study — our team is ready to scope your project and provide a detailed technical proposal.

Proposals typically delivered within 5 business days · NDA available on request